Staff in Jacqui Lambie's office made complaints to her regarding the behaviour of her senior adviser in the months leading up to his dismissal which he is challenging in Federal Court.
Rob Messenger and his wife Fern, who was the senator's former office manager, were sacked in May 2017.
This was about six weeks after they sent a letter to former Prime Minister Malcolm Turnbull detailing complaints over the senator's conduct and workplace health and safety breaches.
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The court on Wednesday heard Senator Lambie discussed concerns over the couple's work performance with two of her staffers in December 2016.
Under questioning from Mr Messenger, office worker Tammy Tyrrell said she told the senator staff had complained about how he treated them and what it was like to work with him.
"Staff felt [Mr Messenger] could be quite intimidating and overpowering," she said.
"Sometimes he spoke over staff and wouldn't listen to responses that were given.
"Basically staff didn't think they were given a fair run.
"They weren't respected, they weren't considered, they weren't taken into account."
Ms Tyrrell described Senator Lambie's reaction to the conversation as one of disbelief.
The court heard evidence of correspondence with Ministerial and Parliamentary Services and Senator Lambie following the discussion in December and a showdown in the office between Mrs Messenger and the senator in February.
Ms Tyrrell said the communication was a result of a breakdown in the relationship between the Messengers and Senator Lambie.
On Tuesday, Mr Messenger had made a request that Justice John Snaden declare her a hostile witness before questioning began.
This was denied.
Justice Snaden on Wednesday advised the evidence he was drawing from Ms Tyrrell was doing him a disservice.
"You are leading evidence from your own witness that is doing, or seeming to do, some damage to your case," he said.