Bob Brown and a further two people were arrested and charged on Wednesday morning while protesting at a forestry site in the Eastern Tiers - the second time he has been arrested at the site in 24 hours.
Tasmania Police confirmed Dr Brown, 75, of Cygnet, was charged with trespass. He was also charged with trespass on Tuesday, and conditionally bailed on both occasions.
Bob Brown Foundation campaign manager Jenny Weber was charged with trespass, along with 72-year-old Kevin Vaughan who also was also charged with wilfully obstruct police.
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Dr Brown slept under a bulldozer last night before police attended again in the morning.
"It was rainy, with temperatures down towards zero, but we were content just to know the swift parrot habitat destruction was slowed by us being there," he said.
He was taken to the Bicheno Police Station and charged.
Dr Brown will appear in the Hobart Magistrates Court on March 31.
His arrest on Tuesday drew criticism from Resources Minister Guy Barnett and the Tasmanian Forest Products Association for allegedly disrupting a worksite.
The protests are targeting the Eastern Tiers, with three STT coupes included in the swift parrot important breeding zone.