Labor leader Rebecca White asked embattled MP David O'Byrne to resign from parliament during Sunday evening's caucus meeting, and will again urge him to resign when parliamentary members meet again this afternoon.
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The party is holding another meeting to discuss the matter, after they were unable to reach a resolution yesterday.
Ms White said the debate was consuming the party and it was time that it ended.
"I hope that, overnight, he has reflected on the comments that were shared with him by his caucus colleagues, and at our party room meeting today he will tender his resignation," she said.
"There's no doubt that my view is very strong on this.
"The community expects leaders like us to uphold certain standards, and I don't believe in this instance that David has upheld those standards.
"Whilst the report found that the behaviour was not a breach of Labor Party policy, as leader of the parliamentary Labor Party, I regard that behaviour to be below the standard that I expect from my members, and that is why I have asked him to resign."
It comes after former Labor premiers Lara Giddings and Paul Lennon also urged Mr O'Byrne to resign, believing his actions over a decade ago - in which he allegedly sexually harassed 22-year-old Rachel Midson while her boss as a union secretary - to be below community standards.
Ms White said the debate had dragged on for long enough and it was clear that Labor Party parliamentarians wanted it resolved.
"This issue continues to fill everybody's media pages, it's on the telly, and it's consuming the Labor Party," she said.
"We need to put an end to this right now, and that's why I have called on David to resign."
Mr O'Byrne earlier stated that the independent report by Barbara Deegan had cleared him, and that comments made earlier by other politicians were "disproportionate, unfair and opportunistic".
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