The St Vincent de Paul Society will close 13 op-shops across the state until April 28 due to social distancing measures associated with fighting the coronavirus pandemic.
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Its store in Queenstown will remain open, however.
The society's chief executive Lara Alexander said the closures were regrettable as many relied on them to shop and socialise.
"Many of these people, including a number of our staff and volunteers, are in the high-risk group so we are mindful of their safety and their welfare," she said.
Ms Alexander said Vinnies would still be able to offer assistance through its emergency assistance, financial counselling, homelessness, mental health, and community housing programs.
She said the charity was in urgent need of cash donations so it could continue to distribute food and cover some living costs for hundreds of individuals and families.
She said $40 could buy food for a family for a week, $25 could buy food for a homeless person, and $100 could cover accommodation for a family for a night.
Donations to Vinnies can be made by visiting www.vinnies.org.au or by calling 13 18 12.