New insignia laws put in place by the state government are aimed at keeping the state safe from organised crime.
This is ahead of a planned memorial ride of the Outlaws on Saturday.
Police Minister Mark Shelton said the laws banned the wearing of insignias for five outlaw motorcycle gangs - Bandidos, Outlaws, Devils Henchmen, Black Uhlans, and Rebels.
"This sends a clear message that crime gangs are not welcome in Tasmania," he said.
Tasmania Police Northern Commander Brett Smith said ahead of the memorial ride on Saturday, police had been in contact with the group to ensure they were fully aware of the new law.
"That includes making sure that they do not wear any of the prescribed items that identifies them as an Outlaw."
Vehicle checks, drug and alcohol testing, monitoring for antisocial and criminal behaviour, and traffic operations are expected to take place at the weekend on the expected route of the ride.
Tasmania Police has also rostered on a significant number of additional officers to assist, and will be supported by contingent of officers from interstate.
"We don't want our community to live in fear because of the behaviour of what these criminal gangs do," Commander Smith said.
"They commit crime, they assault people, and they deal in drugs. They have a culture of violence, and they do not like police.
"I'm pretty confident that just about all of our community would not want that type of behaviour exhibited in our community."
Deputy Labor leader Michelle O'Byrne said more than a year ago, the state government "raced through parliament with significant urgency" the changes to the legislation.
"Nothing has happened around the insignia legislation until in the dark hours of [Wednesday] night," she said.
"If the government have waited until the dark hours of the night to sign a regulation that has not been to parliament, that has not had proper scrutiny, then I'm really not sure what the intent is given Michael Ferguson [former Police Minister] said this was urgent 12 months ago."
Labor voted against the legislation last time it came before the parliament.