Evidence found on the body of murdered tattooist Dwayne Robert Davies links the deceased to the Elderslie residence of his alleged killer, a court has heard.
Mr Davies was last seen on May 26, 2017, and his body was found a week later in a shallow grave at Levendale.
His best friend, Bradley Scott Purkiss, and wife, Margaret Anne Otto, are charged with the alleged murder.
Both have pleaded not guilty.
The Crown alleges former lovers Mr Purkiss and Ms Otto colluded to kill Mr Davies.
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- Accused told police he was being framed for best friend's murder
- Court hears of accused killers' secret affair
- Accused denies knowledge of murder plot
It is alleged Mr Purkiss killed Mr Davies in a cannabis grow shed at his Elderslie residence.
Forensic scientist Ana Flonta told the Hobart Supreme Court on Tuesday twine found on the deceased's clothing was similar to pieces of twine found on the ground of the shed.
"It shows a link between the body of the deceased and the shed," she said.
Ms Flonta attended the cannabis grow shed at Elderslie after the property had been declared a crime scene and later assisted with collecting evidence during the autopsy of Mr Davies.
A blood stain pattern analysis was conducted in the shed.
Ms Flonta said the analysis indicated a "blood-shedding event" happened.
"During this event the blood source was likely close to the ground ... [it was] stationary and bleeding for long period of time," Ms Flonta said.
The court heard swabs were taken of the blood stains which confirmed it was in fact human blood.
Ms Flonta said this was done because "there were a few chicken feathers around."
The blood stains at the shed were consistent with findings made by pathologist Dr Donald Ritchie during the autopsy, Ms Flonta said.
The court heard the stains were consistent with there being no exit wounds.
"Although there is not exit wound, you can still get patterns from the entry wound," Ms Flonta said.
Ms Flonta said an area outside the shed, which tested positive to blood, may have been formed by a dragging motion.
"That is one explanation when it is linear and continuous," she said.
The court heard Ms Flonta tested swabs from dozens of items related to the investigation for blood and samples were also forwarded on to be DNA profiled.
These items include a belt found near the cannabis shed, a towel found in the washing machine at Elderslie, Mr Purkiss' vehicle and twine used to secure Mr Davies' body in a tarp.
Forensic scientist Rita Westbury is expected to give evidence about her DNA findings related to this investigation on Wednesday.
The trial before Chief Justice Alan Blow continues.