Risdon Vale tattooist Dwayne Robert Davies was allegedly killed in a cannabis grow shed on his friend's Elderslie property, a court has heard.
Mr Davies went missing on May 26, 2017 and his body was found just over a week later in a shallow grave at Levendale.
His friend, Bradley Scott Purkiss, of Elderslie, and wife Margaret Anne Otto, of Risdon Vale, are jointly charged with his murder.
Both of the accused have pleaded not guilty.
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The Crown alleged Mr Purkiss lured the victim to his property on the day he went missing and killed him as part of an agreement with Ms Otto.
The court heard in the days following Mr Davies' disappearance, Mr Purkiss' residence was declared a crime scene.
The Crown alleged Mr Davies was killed in a small shed near the main house known as the grow shed, because it was used to produce cannabis.
Tasmania Police crime scene investigator Constable Nick Monk said he photographed a number of blood stains in the shed.
Constable Monk told the court the blood was tested by other forensic investigators and had been found to be a DNA match to the victim.
The jury was shown a walk-through video of the Elderslie property taken by a police officer five days after the alleged murder.
Video of the inside of the grow shed showed several large blood stains on the back wall.
It was alleged by the Crown after Mr Davies was killed Mr Purkiss later dragged his body from the shed and loaded it onto a ute.
Constable Monk said he observed a forensic scientist conduct luminol tests outside the grow shed.
"Luminol is very sensitive to certain compounds and one of those is the iron in blood," Constable Monk said.
The jury was shown photographs taken by Constable Monk of the luminol tests indicating the presence of trace amounts of blood outside the shed.
"I'm fairly certain there weren't any other areas of blood found," Constable Monk said.
Mr Purkiss' defence lawyer Alan Hensley questioned if Constable Monk had observed any indications of a shot blasting through the exterior of the shed.
Constable Monk said he did not see any bullet holes or shell casings, however said there were other officers in charge of searching for such items.
The court heard on Tuesday an autopsy revealed Mr Davies died as the result of being shot twice, in the head and back, with a 12 gauge shotgun.
Constable Monk said he seized from Mr Purkiss' residence six burnt shotgun primers, all 12 gauge, and it was his understanding the primers had been fired.
The trial before Chief Justice Alan Blow continues.