Launceston man Pete McCarron has spent eight months training in hilly areas across the North to prepare for this year's Tour de Cure.
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Mr McCarron will tackle the 1400-kilometre cycle from Sydney to Geelong to raise money for Australian cancer research and treatment advances.
Held from May 3 to 11, this year's cycle includes more than 16,000 metres of climbing, which is about twice the height of Mount Everest.
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Mr McCarron said Queensland last year was a lot flatter, but this year's tour had double the climbing.
The Launcestonian is the only rider representing the state, but he will have the support of fellow Tasmanian Kathy Padgett.
Ms Padgett will be in a support car for the third time, leading the peloton through foreign streets and across state lines.
More than 200 cyclists and support crew will take part in this year's signature tour fundraiser.
Mr McCarron said the event was very close to his heart, with a number of loved ones and close friends diagnosed with cancer in recent years.
"This year over 9000 Tasmanians will be told they have cancer and I want to do my part to help this number be reduced," he said.
"It motivates you when the weather isn't quite nice to go out and train and actually do something.
"We are raising funds to put money into cancer research to hopefully one day get a cure for this insidious disease."
Doing a tour was always on Ms Padgett's bucket list, but she planned to get involved after she'd retired.
"But then a light came on a couple of years ago and I thought 'why wait, just do it now'," she said.
This year's tour will be Ms Padgett's fourth and she said she's addicted.
"I've become part of the core group of drivers," she said.
"We all have the same passion for what we're doing."
Ms Padgett said the participants were like a big family.
"We are all there to raise money to find a cure for cancer," she said.
Mr McCarron and Ms Padgett have been fundraising ahead of the event, with Bunnings BBQs, Oaks Day at Mowbray, raffles and auctions all helping them inch closer to their goal.
As the only Tasmanians involved in the fundraiser, Mr McCarron and Ms Padgett encouraged people to get behind the event.
Donations can be made via tourdecure.com.au.