Brenton Best has failed in his bid to retain his seat in Braddon following the final distribution of preferences today.
The final cut-up saw the Liberals win an unprecedented four seats in the North-West electorate.
It is the first time an electorate has turned in four seats to one party since the parliament was reduced to 25 seats in 1998.
Fellow Liberals Roger Jaensch and Joan Rylah were elected to the two final seats, with Adam Brooks and Jeremy Rockliff already securing their quota earlier in the count.
Former deputy premier Bryan Green won the only other seat in Braddon, with Greens MP Paul O'Halloran also losing his seat.
The Braddon rout handed the Liberals 15 seats in the new parliament.
Mr Best finished with 7583 votes - well behind Mr Jaensch on 9452 and Ms Rylah on 8636.
Former Labor education spokesman Brian Wightman has officially lost his seat as the Tasmanian Electoral Commission finished distributing preferences this morning in the seat of Bass.
Greens MP Kim Booth claimed the fifth and final seat in Bass, 1034 votes ahead of Mr Wightman.
Earlier, fellow Labor MP and former Health Minister Michelle O'Byrne was elected behind the Liberal trio of Peter Gutwein, Sarah Courtney and Michael Ferguson.
In Braddon, the fight for the three final seats is still ongoing with four candidates - Labor's Brenton Best and Bryan Green and the Liberals Roger Jaensch and Joan Rylan - all waiting on the preference cut-up from former Greens MP Paul O'Halloran, who has lost his seat.
On current trends, Mr Best is the most likely to lose his seat.
Labor's Madeleine Ogilvie has pulled 507 votes clear of fellow ALP candidate Julian Amos in the fight for the fifth and final seat in Denison.
Ms Ogilvie has 6811 votes to Mr Amos's 6304 with only Liberal Robert Mallett's 4645 votes left to be distributed.
It is expected that the final make-up of the parliament will be Liberals with 15 seats, Labor seven and the Greens three.